We are pleased to announce a progress in the realisation of the NCN Preludium Bis-1 project (2019/35/O/NZ1/02751) granted to Prof. Ewa Łojkowska. A PhD student, Izabela Perkowska, who won the competition for the scholarship planned as a part of the project, is also involved in realisation of the research tasks. The project concerns the understanding of the role and mechanism of regulation of the expression of genes encoding dioxygenases in the process of plant adaptation to grow and develop on land. It is worth emphasizing that dioxygenases are the main group of enzymes involved in degradation of aromatic compounds.
During the course of the project, the research team has already collected very interesting data on the relationship between the accumulation of coumarins, iron deficiency and the susceptibility of the model plant Arabidopsis thaliana to Dickeya infection.
The following outcomes deserve special attention:
→ optimizing the growth of Arabidopsis thaliana in hydroponic cultures under conditions of abiotic stress (different levels of iron ions) and biotic stress (after inoculation of plants with a suspension of Dickeya sp.).
→ development of the 6-point disease severity scale (DSS)
→ optimization of the method for testing of the expression level of At3g19000, At3g19010 genes in plants inoculated with Dickeya strains
We kindly encourage you to read this research work:
→ Perkowska, I., Potrykus, M., Siwinska, J., Siudem, D., Lojkowska, E., & Ihnatowicz, A. (2021). Interplay between coumarin accumulation, iron deficiency and plant resistance to Dickeya spp. International Journal of Molecular Sciences, 22 (12), 6449.
In addition, the outcomes of the project have been presented so far at two scientific conferences:
• 10th Polish Society of Experimental Plant Biology Virtual Conference, Katowice 20/09/2021 – 23/09/2021. Oral presentation entitled: “Effect of combined environmental stresses on plant health: study using the model system of Arabidopsis thaliana/Dickeya spp. Molecular interactions”
Presentation by Izabela Perkowska; authors: Izabela Perkowska Marta Potrykus, Joanna Siwińska, Dominika Siudem, Ewa Łojkowska, Anna Ihnatowicz
• 31st International Conference on Arabidopsis Research, ICAR2021 Virtual Conference June 21, 2021 – June 25, 2021. Poster presentation: “Effect of iron deficiency on infection symptoms caused by Dickeya spp. in Arabidopsis thaliana” Izabela Perkowska, Marta Potrykus, Joanna Siwińska, Dominika Siudem, Ewa Łojkowska, Anna Ihnatowicz
and during a popularisation of science meeting:
• “Młodzi dla Fahrenheita” – popular science conference (Young for Fahrenheit), Gdańsk, Poland November 17, 2021. Oral presentation entitled: “W służbie roślinom i ludziom. Identyfikacja genów zaangażowanych w biosyntezę roślinnych związków farmakologicznie czynnych” by Izabela Perkowska
