We are pleased to announce a progress in the realisation of the NCN Preludium Bis-1 project (2019/35/O/NZ1/02751) granted to Prof. Ewa Łojkowska. A PhD student, Izabela Perkowska, who won the competition for the scholarship planned as a part of the project, is also involved in realisation of the research tasks besides her accessory supervisor Dr Anna Ihnatowicz. The project concerns the understanding of the role and mechanism of regulation of the expression of genes encoding dioxygenases in the process of plant adaptation to grow and develop on land. It is worth emphasizing that dioxygenases are the main group of enzymes involved in degradation of aromatic compounds.
During the course of the project, the research team has already collected very interesting data on the relationship between the accumulation of coumarins, iron deficiency and the susceptibility of the model plant Arabidopsis thaliana to Dickeya infection.
The following outcomes deserve special attention:
→ optimizing the growth of Arabidopsis thaliana in hydroponic cultures under conditions of abiotic stress (different levels of iron ions) and biotic stress (after inoculation of plants with a suspension of Dickeya sp.).
→ development of the 6-point disease severity scale (DSS)
→ optimization of the method for testing of the expression level of the genes coding dioxygenases in plants inoculated with Dickeya strains
Furthermore MSc Izabela Perkowska performed two interships:
- 09.2023 – 11.2023 (3 months) Laboratoire Agronomie et Environnement, Université de Lorraine INRA – UMT1121, Vandœuvre-lès-Nancy, France.
- 11.2021 (1 month) Laboratoire Agronomie et Environnement, Université de Lorraine INRA – UMT1121, Vandœuvre-lès-Nancy, France
We kindly encourage you to read this research work:
→ Perkowska, I., Potrykus, M., Siwinska, J., Siudem, D., Lojkowska, E., & Ihnatowicz, A. (2021). Interplay between coumarin accumulation, iron deficiency and plant resistance to Dickeya spp. International Journal of Molecular Sciences, 22(12), 6449.
In addition, the outcomes of the project have been presented so far in a form of 9 scientific reports at the conferences:
• 10th Polish Society of Experimental Plant Biology Virtual Conference, Katowice 20.09.2021 –23.09.2021. “Effect of combined environmental stresses on plant health: study using the model system of Arabidopsis thaliana/Dickeya spp. molecular interactions”
Oral presentation by Izabela Perkowska; authors: Izabela Perkowska, Marta
Potrykus, Joanna Siwińska, Dominika Siudem, Ewa Łojkowska, Anna Ihnatowicz
• 31st International Conference on Arabidopsis Research, ICAR2021 Virtual Conference 21.06.2021 – 25.06.2021. Poster “Effect of iron deficiency on infection symptoms caused by Dickeya spp. in Arabidopsis thaliana” by Izabela Perkowska, Marta Potrykus, Joanna Siwińska, Dominika Siudem, Ewa Łojkowska, Anna Ihnatowicz
• Iberian Plant Biology Congress, University of Minho, Braga, Portugal (09–12.07.2023). Poster “Interplay between dioxygenases involved in plant secondary metabolism and drought stress”. by Izabela Perkowska, Alicja Januchowska, Alexandre Olry, Alain Hehn, Ewa Łojkowska, Anna Ihnatowicz
• 3rd PhD Meeting of Plant Science, University of Minho, Braga, Portugal (09.07.2023) “Role of dioxygenases in plant adaptation to oxidative and cold stress responses”. Oral presentation by Izabela Perkowska. Authors: Izabela Perkowska, Alicja Januchowska, Alexandre Olry, Alain Hehn, Ewa Łojkowska, Anna Ihnatowicz
• International Scientific Conference On Plant Biodiversity & Sustainability (online) (13-14.10.2022). “The impact of Arabidopsis thaliana natural populations biodiversity on the content of simple coumarins”. Oral presentation by Izabela Perkowska. Authors: Izabela Perkowska, Joanna Siwińska, Alexandre Orly, Jérémy Grosjean, Alain Hehn, Frédéric Bourgaud, Ewa Łojkowska, Anna Ihnatowicz
• 20th International Symposium on Iron Nutrition & Interactions in Plants ISINIP 2022, Reims, France (04.–08.07.2022). Flash talk “Identification and characterization of Arabidopsis UDP-glucosyltransferase involved in scopolin biosynthesis” by Izabela Perkowska. Authors: Izabela Perkowska, Joanna Siwińska, Alexandre Orly, Jérémy Grosjean, Alain Hehn, Ewa Łojkowska, Anna Ihnatowicz
• 4th International Erwinia Workshop (IEW), Assisi, Italy (02. – 03.07.2022). Poster “Responses of Arabidopsis thaliana plants with disturbed coumarin accumulation to Dickeya dadantii and Dickeya solani infection” by Ewa Łojkowska. Authors: Izabela Perkowska, Marta Potrykus, Joanna Siwinska, Dominika Siudem, Ewa Łojkowska, Anna Ihnatowicz
• Plant Genomes in a Changing Environment 2021 Virtual Conference (18. – 20.10.2021). Lightening talk “Variable profiles of simple coumarins between Arabidopsis thaliana accessions” by Izabela Perkowska. Authors: Izabela Perkowska, Joanna Siwińska, Alexandre Orly, Jérémy Grosjean, Alain Hehn, Frédéric Bourgaud, Ewa Łojkowska, Anna Ihnatowicz
• European Federation of Biotechnology, EFB2021 Virtual Conference (10. – 14.10.2021). Flash poster “Profiling of coumarins in Arabidopsis thaliana accessions using UHPLC-MS” by Izabela Perkowska. Authors: Izabela Perkowska, Joanna Siwińska, Alexandre Orly, Jérémy Grosjean, Alain Hehn, Frédéric Bourgaud, Ewa Łojkowska, Anna Ihnatowicz
and during a popularisation of science meeting:
• “Młodzi dla Fahrenheita” – popular science conference (Young for Fahrenheit), Gdańsk, Poland November 17, 2021. Oral presentation entitled: “W służbie roślinom i ludziom. Identyfikacja genów zaangażowanych w biosyntezę roślinnych związków farmakologicznie czynnych” by Izabela Perkowska
