Progress in realisation of Preludium 21 (2022/45/N/NZ9/01923) project led by Dr. Weronika Babińska-Wensierska.
We are pleased to inform about the progress in the implementation of the project funded by the National Science Centre, Preludium 21 (2022/45/N/NZ9/01923), led by Dr. Weronika Babińska-Wensierska.
The research conducted within the presented project involves a detailed analysis of the soil microbiome in cultivated land, focusing on its impact on the development of diseases such as black leg and soft rot, caused by pectinolytic bacteria from the Dickeya spp. and Pectobacterium spp.
Head of the Preludium 21 project Dr. Weronika Babińska-Wensierska
The research results collected during the first year of the project were presented in oral and poster presentations at national and international conferences:
- Babińska-Wensierska W., Smorawiński K., Motyka-Pomagruk A., Śledź W., Łojkowoska E. “Biodiversity of bacterial plant pathogens from the Pectobacteriaceae family on Polish waterways” 12th International Congress of Plant Pathology, Lyon, France, 20-25.08.2023, poster presentation.
- Babińska-Wensierska W., Motyka-Pomagruk A., Fondi M., Mengoni A., Łojkowska E. “Does the microbiome of soil influence development of blackleg and soft rot diseases caused by Pectobacteriaceae?” Pectobacteriaceae satellite event, Lyon, France, 19-20.08.2023, oral presentation.
- Babińska-Wensierska W., Motyka-Pomagruk A., Fondi A., Mengoni A., Łojkowska E. „Czy microbiom gleby uprawnej wpływa na rozwój chorób czarnej nóżki i mokrej zgnilizny wywoływanych przez bakterie pektynolityczne z rodzaju Dickeya i Pectobacterium?” VII National Microbiological Symposium „Metagenomy różnych środowisk”, Lublin, Poland, 20-21.06.2023, oral presentation.
Importantly, the results obtained within the Preludium 21 project were part of Dr. Weronika Babińska-Wensierska’s doctoral dissertation entitled ” Characterisation of the Pectobacterium aquaticum isolated from Polish water in addition to studying the selected virulence and environmental factors important for development of potato blackleg and soft rot diseases”, supervisor: Prof. Ewa Łojkowska, auxiliary supervisor: Dr. Agata Motyka-Pomagruk.
We will keep you updated on further progress in the implementation of the Preludium 21 project.
Realization of the NCN Preludium Bis-1 project under the supervision of Prof. Ewa Łojkowska
We are pleased to announce a progress in the realisation of the NCN Preludium Bis-1 project (2019/35/O/NZ1/02751) granted to Prof. Ewa Łojkowska. A PhD student, Izabela Perkowska, who won the competition for the scholarship planned as a part of the project, is also involved in realisation of the research tasks besides her accessory supervisor Dr Anna Ihnatowicz. The project concerns the understanding of the role and mechanism of regulation of the expression of genes encoding dioxygenases in the process of plant adaptation to grow and develop on land. It is worth emphasizing that dioxygenases are the main group of enzymes involved in degradation of aromatic compounds.
During the course of the project, the research team has already collected very interesting data on the relationship between the accumulation of coumarins, iron deficiency and the susceptibility of the model plant Arabidopsis thaliana to Dickeya infection.
The following outcomes deserve special attention:
→ optimizing the growth of Arabidopsis thaliana in hydroponic cultures under conditions of abiotic stress (different levels of iron ions) and biotic stress (after inoculation of plants with a suspension of Dickeya sp.).
→ development of the 6-point disease severity scale (DSS)
→ optimization of the method for testing of the expression level of the genes coding dioxygenases in plants inoculated with Dickeya strains
Furthermore MSc Izabela Perkowska performed two interships:
- 09.2023 – 11.2023 (3 months) Laboratoire Agronomie et Environnement, Université de Lorraine INRA – UMT1121, Vandœuvre-lès-Nancy, France.
- 11.2021 (1 month) Laboratoire Agronomie et Environnement, Université de Lorraine INRA – UMT1121, Vandœuvre-lès-Nancy, France
We kindly encourage you to read this research work:
→ Perkowska, I., Potrykus, M., Siwinska, J., Siudem, D., Lojkowska, E., & Ihnatowicz, A. (2021). Interplay between coumarin accumulation, iron deficiency and plant resistance to Dickeya spp. International Journal of Molecular Sciences, 22(12), 6449.
In addition, the outcomes of the project have been presented so far in a form of 9 scientific reports at the conferences:
• 10th Polish Society of Experimental Plant Biology Virtual Conference, Katowice 20.09.2021 –23.09.2021. “Effect of combined environmental stresses on plant health: study using the model system of Arabidopsis thaliana/Dickeya spp. molecular interactions”
Oral presentation by Izabela Perkowska; authors: Izabela Perkowska, Marta
Potrykus, Joanna Siwińska, Dominika Siudem, Ewa Łojkowska, Anna Ihnatowicz
• 31st International Conference on Arabidopsis Research, ICAR2021 Virtual Conference 21.06.2021 – 25.06.2021. Poster “Effect of iron deficiency on infection symptoms caused by Dickeya spp. in Arabidopsis thaliana” by Izabela Perkowska, Marta Potrykus, Joanna Siwińska, Dominika Siudem, Ewa Łojkowska, Anna Ihnatowicz
• Iberian Plant Biology Congress, University of Minho, Braga, Portugal (09–12.07.2023). Poster “Interplay between dioxygenases involved in plant secondary metabolism and drought stress”. by Izabela Perkowska, Alicja Januchowska, Alexandre Olry, Alain Hehn, Ewa Łojkowska, Anna Ihnatowicz
• 3rd PhD Meeting of Plant Science, University of Minho, Braga, Portugal (09.07.2023) “Role of dioxygenases in plant adaptation to oxidative and cold stress responses”. Oral presentation by Izabela Perkowska. Authors: Izabela Perkowska, Alicja Januchowska, Alexandre Olry, Alain Hehn, Ewa Łojkowska, Anna Ihnatowicz
• International Scientific Conference On Plant Biodiversity & Sustainability (online) (13-14.10.2022). “The impact of Arabidopsis thaliana natural populations biodiversity on the content of simple coumarins”. Oral presentation by Izabela Perkowska. Authors: Izabela Perkowska, Joanna Siwińska, Alexandre Orly, Jérémy Grosjean, Alain Hehn, Frédéric Bourgaud, Ewa Łojkowska, Anna Ihnatowicz
• 20th International Symposium on Iron Nutrition & Interactions in Plants ISINIP 2022, Reims, France (04.–08.07.2022). Flash talk “Identification and characterization of Arabidopsis UDP-glucosyltransferase involved in scopolin biosynthesis” by Izabela Perkowska. Authors: Izabela Perkowska, Joanna Siwińska, Alexandre Orly, Jérémy Grosjean, Alain Hehn, Ewa Łojkowska, Anna Ihnatowicz
• 4th International Erwinia Workshop (IEW), Assisi, Italy (02. – 03.07.2022). Poster “Responses of Arabidopsis thaliana plants with disturbed coumarin accumulation to Dickeya dadantii and Dickeya solani infection” by Ewa Łojkowska. Authors: Izabela Perkowska, Marta Potrykus, Joanna Siwinska, Dominika Siudem, Ewa Łojkowska, Anna Ihnatowicz
• Plant Genomes in a Changing Environment 2021 Virtual Conference (18. – 20.10.2021). Lightening talk “Variable profiles of simple coumarins between Arabidopsis thaliana accessions” by Izabela Perkowska. Authors: Izabela Perkowska, Joanna Siwińska, Alexandre Orly, Jérémy Grosjean, Alain Hehn, Frédéric Bourgaud, Ewa Łojkowska, Anna Ihnatowicz
• European Federation of Biotechnology, EFB2021 Virtual Conference (10. – 14.10.2021). Flash poster “Profiling of coumarins in Arabidopsis thaliana accessions using UHPLC-MS” by Izabela Perkowska. Authors: Izabela Perkowska, Joanna Siwińska, Alexandre Orly, Jérémy Grosjean, Alain Hehn, Frédéric Bourgaud, Ewa Łojkowska, Anna Ihnatowicz
and during a popularisation of science meeting:
• “Młodzi dla Fahrenheita” – popular science conference (Young for Fahrenheit), Gdańsk, Poland November 17, 2021. Oral presentation entitled: “W służbie roślinom i ludziom. Identyfikacja genów zaangażowanych w biosyntezę roślinnych związków farmakologicznie czynnych” by Izabela Perkowska
Realization of the NCN Opus 17 project led by Dr. Wojciech Śledź
We would like to keep you informed about the progress in realization of OPUS 17 project (2019/33/B/NZ9/00940) granted by the National Science Center in Poland.
Fruitful cooperation with the research team of Dr. eng. Anna Patrycja Dzimitrowicz Prof. PWr from Wrocław University of Science and Technology, contributed to dissemination of the research results in the form of 5 scientific publications:
Collaborators in the OPUS 17 project (from left): Prof. Paweł Pohl, Dr. eng. Anna Dzimitrowicz prof. PWr (head of the project from PWr), Dr. eng. Piotr Jamróz prof. PWr, Dr. Agata Motyka-Pomagruk, Dr. eng. Wojciech Śledź (head of the project).
→ Dzimitrowicz, Anna, Piotr Jamroz, Pawel Pohl, Weronika Babinska, Dominik Terefinko, Wojciech Sledz, and Agata Motyka-Pomagruk. “Multivariate optimization of the FLC-dc-APGD-based reaction-discharge system for continuous production of a plasma-activated liquid of defined physicochemical and anti-phytopathogenic properties.” International Journal of Molecular Sciences 22, no. 9 (2021): 4813.
→ Motyka-Pomagruk, Agata, Anna Dzimitrowicz, Jakub Orlowski, Weronika Babinska, Dominik Terefinko, Michal Rychlowski, Michal Prusinski et al. “Implementation of a non-thermal atmospheric pressure plasma for eradication of plant pathogens from a surface of economically important seeds.” International Journal of Molecular Sciences 22, no. 17 (2021): 9256.
→ Babinska, Weronika, Agata Motyka-Pomagruk, Wojciech Sledz, Agnieszka Kowalczyk, Zbigniew Kaczynski, and Ewa Lojkowska. “The first Polish isolate of a novel species Pectobacterium aquaticum originates from a Pomeranian Lake.” International Journal of Environmental Research and Public Health 18, no. 9 (2021): 5041.
→ Dzimitrowicz, Anna, Pawel Pohl, Magda Caban, Piotr Jamroz, Piotr Cyganowski, Mateusz Bykowski, Aleksandra Klimczak, and Aleksandra Bielawska-Pohl. “How does direct current atmospheric pressure glow discharge application influence on physicochemical, nutritional, microbiological, and cytotoxic properties of orange juice?.” Food Chemistry 377 (2022): 131903.
→ Śledź Wojciech, Motyka-Pomagruk Agata, Żukowska Dominika, Babińska‑Wensierska Weronika, Żołędowska Sabina, Łojkowska Ewa. Genotypic and phenotypic uniformity among the population of Pectobacterium atrosepticum strains isolated during three growing seasons from potato fields in Poland. European Journal of Plant Pathology 2023, 167(1): 99-121 (doi: 10.1007/s10658-023-02687-y).
And two patents and two more patent applications:
→ ,,Sposób eradykacji bakteryjnych fitopatogenów oraz przepływowy układ reakcyjno-wykładowczy do realizacji tego sposobu”. Numer prawa wyłącznego Pat. 236055 z dnia 2020-07-24. Twórcy: Łojkowska Ewa, Śledź Wojciech, Motyka Agata, Anna Dzimitrowicz, Piotr Jamróz, Paweł Pohl. Zgłaszający: Politechnika Wrocławska i Uniwersytet Gdański.
→ ,,Sposób otrzymania preparatu do stymulacji wzrostu roślin, preparat otrzymany tym sposobem oraz zastosowanie preparatu do stymulacji wzrostu roślin, w szczególności tych istotnych gospodarczo”. Numer prawa wyłącznego Pat.236377 z dnia 2020-03-17. Twórcy: Agata Motyka-Pomagruk, Anna Dzimitrowicz, Weronika Babińska, Wojciech Śledź, Paweł Pohl, Piotr Jamróz, Dominik Terefinko, Ewa Łojkowska. Zgłaszający: Politechnika Wrocławska i Uniwersytet Gdański.
→ ,,Sposób ochrony roślin istotnych gospodarczo, zwłaszcza roślin naczyniowych, przed patogenami bakteryjnymi” Zgłoszenie patentowe (UP RP) nr P.438360 z dnia 2021-07-06. Twórcy: Dzimitrowicz Anna, Jamróz Piotr, Pohl Paweł, Terefinko Dominik, Motyka-Pomagruk Agata, Babińska Weronika, Śledź Wojciech, Łojkowska Ewa, Orłowski Jakub, Prusiński Michał. Zgłaszający: Politechnika Wrocławska i Uniwersytet Gdański.
→ ,,Method for producing a formulation of antibacterial properties, the formulation obtained by this method and its application in addition to a flow-through reaction-discharge system for implementing this method” Zgłoszenie patentowe (UP RP) nr P.447139 z 2023-12-18. Twórcy: Dzimitrowicz Anna, Motyka-Pomagruk Agata, Terefinko Dominik, Jamróz Piotr, Śledź Wojciech, Orłowski Jakub, Babińska-Wensierska Weronika, Paweł Pohl, Łojkowska Ewa. Zgłaszający: Politechnika Wrocławska i Uniwersytet Gdański.
It is worth emphasizing that Dr. Agata Motyka-Pomagruk, who presented the results of the OPUS 17 project in the Young Fahrenheit competition (2023), won the First Prize, which indicates the high commercialization potential of the collected research results.
We will inform you about the next successes🙂
Realisation of NCN Sonata 15 project (2019/35/D/ST8/04107)
We would like to inform you about the progress made in the realisation of the Sonata 15 project (2019/35/D/ST8/04107), which was granted by the National Science Center to Wroclaw University of Science and Technology (Leader) in a consortium with University of Gdańsk.
The head of the project from the Leader side is Dr. hab. Anna Patrycja Dzimitrowicz Prof. PWr from Wrocław University of Science and Technology, and from the partner part, Dr. Eng. Wojciech Śledź. Our fruitful cooperation has resulted in dissemination of the research results in the form of numerous conference reports and so far six scientific publications:
Head of the Sonata 15 project Dr. hab. eng. Anna Dzimitrowicz (from the left) and Dr. Agata Motyka-Pomagruk (from the right)
→ Dzimitrowicz, Anna, Agata Motyka-Pomagruk, Piotr Cyganowski, Piotr Jamroz, Dominik Terefinko, Pawel Pohl, Tymoteusz Klis, and Magda Caban. “Cold atmospheric pressure plasmas as versatile tools for effective degradation of a mixture of hazardous and endocrine disturbing compounds from liquid wastes.” Journal of Environmental Chemical Engineering 9, no. 6 (2021): 106718.
→ Dzimitrowicz, Anna, Magda Caban, Dominik Terefinko, Pawel Pohl, Piotr Jamroz, Weronika Babinska, Piotr Cyganowski et al. “Application of pulse-modulated radio-frequency atmospheric pressure glow discharge for degradation of doxycycline from a flowing liquid solution.” Scientific reports 12, no. 1 (2022): 1-16.
→ Terefinko Dominik, Caban Magda, Motyka-Pomagruk Agata, Babińska Weronika, Pohl Paweł, Jamróz Piotr, Cyganowski Piotr, Śledź Wojciech, Łojkowska Ewa, Stepnowski Piotr, Dzimitrowicz Anna. Removal of clinically significant antibiotics from aqueous solutions by applying unique high-throughput continuous-flow plasma pencil and plasma brush systems. Chemical Engineering Journal 452 (2023): 139415. (doi: 10.1016/j.cej.2022.139415).
→ Dzimitrowicz Anna, Dominik Terefinko, Aleksandra Bielawska-Pohl, Agata Motyka-Pomagruk, Piotr Jamroz, Piotr Cyganowski, Karina Lenard, Pawel Pohl, Aleksandra Klimczak, and Magda Caban. Biosafe removal of diclofenac from wastewaters by a continuous-flow mode cold atmospheric pressure plasma system. Journal of Environmental Chemical Engineering 12, 1 (2024): 111598.
→ Mujahid Ameen Khan, Anna Dzimitrowicz, Magda Caban, Piotr Jamroz, Dominik Terefinko, Włodzimierz Tylus, Pawel Pohl Piotr Cyganowski. Catalytically enhanced direct degradation of nitro-based antibacterial agents using dielectric barrier discharge cold atmospheric pressure plasma and rhenium nanoparticles. Environmental Research (2023): 116297.
→ Cyganowski, Piotr, Magda Caban, Mujahid Ameen Khan, Mateusz M. Marzec, Andrzej Zak, Pawel Pohl, Piotr Jamroz, Andrzej Bernasik, and Anna Dzimitrowicz. “The use of cold atmospheric pressure plasma for the synthesis of saccharide-stabilized Re nanostructures enabling effective deactivation of nitro-based antimicrobial agents.” Plasma Chemistry and Plasma Processing 43, no. 1 (2023): 199-224.
In the course of the project, we also prepared 6 patent applications, including 2 international in the PCT and EPO procedures:
→ „Method of degrading antibiotics from aqueous solutions by using cold atmospheric pressure plasma generated in a flowing plasma brush and a plasma brush intended for this method.” Zgłoszenie patentowe międzynarodowe tryb PCT nr PCT/PL2022/050027 z dnia 2022-05-06. Twórcy: Anna Dzimitrowicz, Agata Motyka-Pomagruk, Magda Caban, Weronika Babińska, Dominik Terefinko, Wojciech Śledź, Piotr Jamróz, Piotr Cyganowski, Ewa Łojkowska, Piotr Stepnowski, Paweł Pohl. Zgłaszający: Politechnika Wrocławska i Uniwersytet Gdański.
→ ,,Method for eradication of pathogenic microorganisms from flat surfaces or skin tissue and a system for carrying out this method” Zgłoszenie patentowe międzynarodowe tryb EPO nr. EP23169144.5. z 2023-04-21. Twórcy: Motyka-Pomagruk Agata, Śledź Wojciech, Prusiński Michał, Orłowski Jakub, Babińska Weronika, Łojkowska Ewa, Dzimitrowicz Anna, Jamróz Piotr, Pohl Paweł, Jerzy Dora. Zgłaszający: Wroclaw University of Science and Technology and University of Gdansk
→ ,,Sposób eradykacji drobnoustrojów chorobotwórczych z powierzchni płaskich lub tkanki skórnej oraz układ do realizacji tego sposobu” Zgłoszenie patentowe (UP RP) nr. P.441356 z dnia 2022-06-02. Twórcy: Motyka-Pomagruk Agata, Śledź Wojciech, Prusiński Michał, Orłowski Jakub, Babińska Weronika, Łojkowska Ewa, Dzimitrowicz Anna, Jamróz Piotr, Pohl Paweł. Zgłaszający: Politechnika Wrocławska i Uniwersytet Gdański.
→ ,,Sposób dezaktywacji związków endokrynnie czynnych z roztworów wodnych” Zgłoszenie patentowe (UP RP) nr P.438832 z dnia 2021-08-27. Twórcy: Dzimitrowicz Anna, Śledź Wojciech, Caban Magda, Jamróz Piotr, Motyka-Pomagruk Agata, Cyganowski Piotr, Terefinko Dominik, Stepnowski Piotr, Pohl Paweł. Zgłaszający: Politechnika Wrocławska i Uniwersytet Gdański.
→ ,,Sposób degradacji antybiotyków z roztworów wodnych z zastosowaniem nietermicznej plazmy atmosferycznej” Zgłoszenie patentowe (UP RP) nr P.437603 z dnia 2021-04-17. Twórcy: Dzimitrowicz Anna, Śledź Wojciech, Caban Magda, Jamróz Piotr, Motyka-Pomagruk Agata, Babińska Weronika, Cyganowski Piotr, Terefinko Dominik, Stepnowski Piotr, Pohl Paweł, Łojkowska Ewa. Zgłaszający: Politechnika Wrocławska i Uniwersytet Gdański.
→ „Sposób rozkładu antybiotyków z roztworów wodnych z zastosowaniem zimnej plazmy atmosferycznej, generowanej w przepływowej szczotce plazmowej oraz szczotka plazmowa do realizacji tego sposobu” Zgłoszenie patentowe (UP RP) nr P.440185 z dnia 2022-01-20. Twórcy: Anna Dzimitrowicz, Agata Motyka-Pomagruk, Magda Caban, Weronika Babińska, Dominik Terefinko, Wojciech Śledź, Piotr Jamróz, Piotr Cyganowski, Ewa Łojkowska, Piotr Stepnowski, Paweł Pohl. Zgłaszający: Politechnika Wrocławska i Uniwersytet Gdański.
It is worth noting that the idea of degradation of antibiotics with the use of cold atmospheric plasmas developed in the Sonata 15 project won the Main Prize in the Eureka! DGP competition (2022) and granted to Weronika Babińska-Wensierska 3rd Award in Young Fahrenheit competition (2024).
Dr. eng. Wojciech Śledź – head from the partner side of Sonata 15 (in front). Prof. Paweł Pohl (in the back).
Head of the Sonata 15 project Dr. hab. eng. Anna Dzimitrowicz (from the left) and Dr. Agata Motyka-Pomagruk (from the right)
We will inform you about the next successes in the course of the project realisation 🙂
Our scope of accredition has been successfully extended to include microbiological analyses of water samples
Our research group involving:
- Professor Ewa Łojkowska
- Dr. Wojciech Śledź
- Dr Agata Motyka-Pomagruk
- Dr Natalia Kaczyńska
- MSc Weronika Babińska-Wensierska
has successfully enlarged the scope of accreditation of Research & Development Laboratory of IFB UG & MUG to include microbiological analyses of drinkable water samples in terms of total microbial count (at 22°C and 36°C) according to standard PN-EN ISO 6222:2004, the number of fecal enterococci by membrane filtration according to PN-EN 7899-2:2004 in addition to the number of Escherichia coli and the number of the members of coli group by membrane filtration according to PN-EN ISO 9308-1:2014-12/A1:2017-04.
We highly recommend ordering analyses based on these methodologies in our laboratory:)
Photo generated with AI (at https://perchance.org/ai-photo-generator)
Weronika Babińska-Wensierska was granted with 3rd Award in the Young Fahrenheit Competition
We are pleased to announce that Weronika Babińska-Wensierska was granted with 3rd Award in the third edition of Young Fahrenheit competition. The invention of the laureate and her collaborates is a pro-ecological method based on the use of cold atmospheric pressure plasmas, in the form of plasma pencil and plasma brush, for degradation of antibiotics residuals from liquid waste.
The main objective of the Young Fahrenheit competition, organized by Univentum Labs in cooperation with the Center for Technology Transfer of the University of Gdańsk, is to search for inventions of a commercialization potential.
Our scope of accredition has been successfully extended to an elastic one in terms of PCR, multiplex PCR and the culture methods
Our research group involving:
- Professor Ewa Łojkowska
- Dr. Wojciech Śledź
- Dr Agata Motyka-Pomagruk
- Dr Natalia Kaczyńska
- MSc Weronika Babińska-Wensierska
has successfully enlarged the scope of accreditation of Research & Development Laboratory of IFB UG & MUG to an elastic one in terms of PCR, multiplex PCR and the culture methods. We highly recommend ordering analyses based on these methodologies in our laboratory:)
We search for laboratories willing to participate in interlaboratory comparisons with us
We are searching for laboratories that would like to participate with us in interlaboratory comparisons in terms of detection of soft rot Pectobacteriaceae. For further details please contact our specialists:
The research group of Laboratory of Research and Development at UG and MUG. From the left: Dr. Agata Motyka-Pomagruk, Dr. Natalia Kaczyńska, MSc Weronika Babińska-Wensierska, Dr. eng. Wojciech Śledź, Prof. Ewa Łojkowska, MSc Michał Prusiński
Our research group has been honoured with a Rector’s Award
Our research group involving:
- Professor Ewa Łojkowska
- Dr. Wojciech Śledź
- Dr Agata Motyka-Pomagruk
- Dr Natalia Kaczyńska
has been honoured with a Rector’s Award for publications and inventions resulting from our research activities in 2022.
Also PhD student from our group, MSc Weronika Babińska-Wensierska has been awarded with a Rector’s price for PhD students for her individual impact into science.
The research group of Laboratory of Research and Development at UG and MUG. From the left: Dr. Agata Motyka-Pomagruk, Dr. Natalia Kaczyńska, MSc Weronika Babińska-Wensierska, Dr. eng. Wojciech Śledź, Prof. Ewa Łojkowska, MSc Michał Prusiński