DATE: to be announced PRICE: to be announced
Molecular cloning (basic course)
Who is invited?
The course is dedicated to employees of universities, research institutes, inspections, companies from the R&D sector, as well as students of medical or natural sciences who want to gain knowledge and expand their research workshop with tools from the field of molecular biology.
Programme
The course will familiarize participants with the methods of DNA cloning. Both theoretical and practical knowledge of DNA isolation techniques, polymerase chain reaction (PCR), methods of cloning genes into plasmid vectors will be gained during this course. The detailed programme:
Day I
- Theoretical introduction (selection of vector and host organism)
- Isolation and purification of plasmid DNA from bacteria
- Preparation of the vector and the insert: digestion of plasmid DNA with restriction endonucleases, DNA electrophoresis in agarose gel, isolation and purification of a DNA fragment from agarose gel
Day II
- Obtaining a recombinant DNA molecule: ligation
- Introduction of a recombinant DNA molecule into a bacterial cell: transformation (selection of transformants)
Day III
- Analysis of transformants for the presence of recombinant DNA molecule: lysis of bacterial cells and polymerase chain reaction (colony PCR), DNA electrophoresis in agarose gel
General Information
The training will be conducted in small groups (up to 8 people) in the Research & Development Laboratory of IFB UG & MUG (20 Podwale Przedmiejskie street, 80-824 Gdańsk, Poland). Each participant will conduct all the experiments on his own in order to obtain the necessary laboratory practice. The courses will be conducted by experienced academia teachers, specialists and practitions in the field. The participants will receive certificates confirming acquisition of the practical skills.
If you need a specialized course from the field of molecular biology, microbiology, ISO standards, bioinformatics or chemistry feel free to contact our team. We collaborate with specialists from various fields and may prepare an individual offer for your group. Contact details:
dr Agata Motyka-Pomagruk (agata.motyka-pomagruk@ug.edu.pl; +48 606 406 058)
dr Wojciech Śledź (wojciech.sledz@biotech.ug.edu.pl; +48 603 297 737)
*VAT exemption
In the case of participation in the training of an employee raising professional qualifications and financing from public funds, it is possible to obtain a tax exemption after providing an appropriate statement. The statement should be signed by an authorized person. The scanned statement should be sent during registration for the course; the original can be delivered on the start date.